HISTONE SPECIFICITY REVEALED BY AMMONIACAL SILVER STAINING
نویسندگان
چکیده
منابع مشابه
Improved mass spectrometry compatibility is afforded by ammoniacal silver staining.
Sequence coverage in MS analysis of protein digestion-derived peptides is a key issue for detailed characterization of proteins or identification at low quantities. In gel-based proteomics studies, the sequence coverage greatly depends on the protein detection method. It is shown here that ammoniacal silver detection methods offer improved sequence coverage over standard silver nitrate methods,...
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Paraffin sections were deparaffinized and hydrated to distilled water, then placed in 20% silver nitrate and kept in 60°C for 15 minutes, then rinsed in distilled water. Then sections were treated with formalin solution containing ammoniacal silver solution for about 15 min, followed by rinsing and placing tissue in sodium thiosulfate solution for 2 minutes and washing in tap water. The section...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1966
ISSN: 0022-1554,1551-5044
DOI: 10.1177/14.2.177